Conclusion: Strain LS3K can tolerate ammonium at 756.77 mM and has the ability for growing in animals manure contained a medium that should make it applicable for use in animal waste handling systems as well as for enhancing the capability of deodorization. This bacterium has an optimal growth temperature 30☌. Colonies (l-2 mm in diameter) on plate culture are yellowish, circular and smooth. The cells of strain LS3K are short rods, aerobic and motile. The 16S rRNA gene sequence analysis demonstrated that the isolate is showing high similarity to species of Pseudomonas tolaasii. The content of DNA G+C of the strain was 55.2%. Based on the morphology, physiological observation and the 16S rRNA gene sequence, strain LS3T was identified as Pseudomonas spp. Results: High ammonium sulfate-responsive, strain LS3K was successfully isolated and identified based on morphology, physiology and biochemical identifications, as well as molecular identification based on 16S rRNA sequence. The reduction of ammonium was measured from cultured medium using nessler reagent spectroscopically. Selected strain was then identified based on morphology and biochemical identifications, as well as molecular identification. The isolation was using a 1/100 nutrient agar with high concentration of (NH 4) 2SO 4 as an organic ammonium stressor. Materials and Methods: The strain is originally obtained from soil sample collected at ammonia high-emitted of chicken hen production area at tropical country Indonesia. This study was performed to identify strains bacteria screened from the soil in the odorous region of apoultry format Yogyakarta city, Indonesia, to evaluate their capability for growing in ammonium and animals urine high content medium and also to investigate the ability to reduce ammonium concentration from the liquid medium. Background and Objective: A proper management processes for safe and efficient handling of animal manure is important to minimize the possibilities of global environmental problem due to the emission of unpleasant odor.

Sequencher stored at room temperature